Advantages of low-code in Intranet Portals: Enhancing the visualization of internal data in a major retail chain through low-code applications

Internship Report presented as the partial requirement for obtaining a Master's degree in Information Management, specialization in Information Systems and Technologies ManagementLIDL, a major multinational retail chain with branches spread across the globe, has many internal processes that aren’t centralized. In each country branch, information is dispersed within several platforms, in different formats, which in turn makes data harder to analyze, slowing down procedures that are frequently used within each division of the major retail chain. With this problem in mind LIDL has decided to invest in Low-Code, giving the liberty to each country to develop its own internal portal to counter this problem. With this, each branch centralizes all its essential information in one place. By choosing low-code, LIDL has given each country the freedom of developing the necessary applications in record time, providing a way to experience omnichannel experiences without giant budgets and costly development teams. The results of this study show why portal development should be done with Low-Code, the synergy that is built between the two concepts and the many advantages that follow. To defend these claims, the work done during my internship will be showcased and analyzed

Sustentabilidade da montanha portuguesa: realidades

Neste capítulo introdutório faz-se uma contextualização dos sistemas de montanha no mundo e em Portugal. Apresentam-se definições de montanha e suas aplicações bem como se descreve a distribuição destes sistemas. Caracterizam-se os elementos distintivos dos sistemas de montanha com base em variáveis como o clima, a altitude e o relevo, geologia, biodiversidade, culturas agrícolas e florestais e ainda em aspetos socioeconómicos. São ainda discutidas as razões pelas quais as montanhas são importantes para as sociedades, o que inclui principalmente processos e funções relacionados com o fornecimento de serviços de ecossistema como a conservação da biodiversidade e a regulação de processos atmosféricos e hidrológicos, a maior parte dos quais insubstituíveis. O capítulo termina com uma breve análise da investigação conduzida em espaços de montanha em Portugal.info:eu-repo/semantics/publishedVersio

Brucella spp noncanonical LPS: structure, biosynthesis, and interaction with host immune system

Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in humans. Several species are recognized within the genus Brucella and this classification is mainly based on the difference in pathogenicity and in host preference. Brucella strains may occur as either smooth or rough, expressing smooth LPS (S-LPS) or rough LPS (R-LPS) as major surface antigen. This bacterium possesses an unconventional non-endotoxic lipopolysaccharide that confers resistance to anti-microbial attacks and modulates the host immune response. The strains that are pathogenic for humans (B. abortus, B. suis, B. melitensis) carry a smooth LPS involved in the virulence of these bacteria. The LPS O-chain protects the bacteria from cellular cationic peptides, oxygen metabolites and complement-mediated lysis and it is a key molecule for Brucella survival and replication in the host. Here, we review i) Brucella LPS structure; ii) Brucella genome, iii) genes involved in LPS biosynthesis; iv) the interaction between LPS and innate immunity

Recombinant Lactococcus lactis fails to secrete bovine chymosine

Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for heterologous chymosin production for the following reasons: (1) it is considered to be a GRAS (generally regarded as safe) microorganism, (2) only one protease is present on its surface, (3) it can secrete proteins of different sizes, and (4) it allows for the direct production of protein in fermented food products. Thus, three genetically modified L. lactis strains were constructed to produce and target the three different forms of bovine chymosin, prochymosin B, chymosin A and chymosin B to the extracellular medium. Although all three proteins were stably produced in L. lactis, none of the forms were detected in the extracellular medium or showed clotting activity in milk. Our hypothesis is that this secretion deficiency and lack of clotting activity can be explained by the recombinant protein being attached to the cell envelope. Thus, the development of other strategies is necessary to achieve both production and targeting of chymosin in L. lactis, which could facilitate the downstream processing and recovery of this industrially important protein.Fil: Diniz Luerce, Tessália. Federal University of Minas Gerais. Institute of Biological Sciences. Department of General Biology; BrasilFil: Santiago Pacheco Azevedo, Marcela. Federal University of Minas Gerais. Institute of Biological Sciences. Department of General Biology; BrasilFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Azevedo, Vasco. Federal University of Minas Gerais. Institute of Biological Sciences. Department of General Biology; BrasilFil: Miyoshi, Anderson. Federal University of Minas Gerais. Institute of Biological Sciences. Department of General Biology; BrasilFil: Santos Pontes, Daniela. State University of Paraiba. Centre of Biological and Applied Social Sciences; Brasi

Esquizofrénicos em medida de internamento: análise diferencial das características e do risco de violência de homicidas e não homicidas

Há evidências empíricas de que as pessoas com doença mental têm maiorprobabilidade de cometer uma ofensa criminal do que as pessoas sem qualquer perturbação ede que a probabilidade dos esquizofrénicos cometerem homicídios é superior à de outrosdoentes psicóticos e da população em geral. Acresce que existe atualmente uma forteevidência da associação entre esquizofrenia, o risco de cometer uma ofensa violenta,especialmente homicídio, e a comorbilidade com o abuso de substâncias. Contudo, osofensores com perturbações mentais têm menor probabilidade de reincidir do que osofensores sem perturbações mentais, apesar de existir uma semelhança entre os principaisfatores de risco de reincidência entre ambos. A avaliação do risco de reincidência criminal é,neste contexto, relevante, especialmente dada a questão da eventual continuidade injustificadade medidas de segurança de Internamento.Empirical evidence demonstrates that people who develop major mental disorders aremore likely of committing any criminal offense than persons without these disorders, and thatschizophrenics are more likely to commit homicide than other psychotics and the generalpopulation. Also, there is now robust evidence demonstrating the association betweenschizophrenia, the risk of committing a violent offending, specially homicide, and thepresence of co-occurring substance abuse disorder. Yet, mentally disordered offenders are lesslikely to recidivate than nondisordered offenders, although the predictors of recidivism arealmost identical in both groups. Criminal reoffending risk assessment is relevant, especiallybecause this rise the question of the eventual unjustified confinement of mentally disorderedoffenders

Therapeutic DNA Vaccines: The Final Step for Success

Therapeutic DNA vaccines are mostly plasmidic constructs containing a strong promoter that allows in situ transcription and translation of one or many encoded proteins/antigens to induce protective cellular and humoral immune responses against different pathogenic organisms [1–5]. Currently, at least 114 open clinical studies are recruiting patients for distinct clinical phases using a DNAvaccine approach

Development of photocatalytic ceramic materials through the deposition of TiO2 nanoparticles layers

Urbanism and communities centralization enlarges atmospheric pollution that affects both human beings as well as their constructed buildings. Different scientific and technological studies are being conducted, both in academic and construction industry, aiming the development of new construction materials with properties that can decrease visual pollution of cities, reducing also the number of cleanings required. The present research work aims the study and the production of self-cleaning ceramic surfaces in an economical and viable way without changing aesthetical aspect of material substrates used. The use of TiO2 nanoparticles (TiO2-NNPs) represents an attractive way to generate self-cleaning surfaces, therefore promoting the degradation of pollutant agents and reducing cleaning maintenance costs. In order to impart self-cleaning properties to ceramic surfaces, TiO2-NNPs based layers were deposited on different ceramic material substrates using the dip-coating method. The Photocatalytic activity (degradation of pollutants adsorbed on the surface) of the TiO2-NNPs based layers was characterized via the decomposition rate of an aqueous solution of Methylene blue (MB) under UV light irradiation. Colourless layers were successfully produced onto gray and white ceramic substrates using this sol-gel technique, without changing their aesthetical appearance. It was observed that the best photocatalytic activity was exhibited by the most porous ceramic substrate (gray); nevertheless, all the TiO2-NNPs coated ceramic surfaces showed good photocatalytic efficiency.Revigres® Compan

Current review of genetically modified lactic acid bacteria for the prevention and treatment of colitis using murine models

Inflammatory Bowel Diseases (IBD) are disorders of the gastrointestinal tract characterized by recurrent inflammation that requires lifelong treatments. Probiotic microorganisms appear as an alternative for these patients; however, probiotic characteristics are strain dependent and each probiotic needs to be tested to understand the underlining mechanisms involved in their beneficial properties. Genetic modification of lactic acid bacteria (LAB) was also described as a tool for new IBD treatments.The first part of this review shows different genetically modified LAB (GM-LAB) described for IBD treatment since 2000.Then, the two principally studied strategies are discussed (i) GM-LAB producing antioxidant enzymes and (ii) GM-LAB producing the anti-inflammatory cytokine IL-10. Different delivery systems, including protein delivery and DNA delivery, will also be discussed. Studies show the efficacy of GM-LAB (using different expression systems) for the prevention and treatment of IBD, highlighting the importance of the bacterial strain selection (with anti-inflammatory innate properties) as a promising alternative. These microorganisms could be used in the near future for the development of therapeutic products with anti-inflammatory properties that can improve the quality of life of IBD patients.Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; FranciaFil: Miyoshi, Anderson. Universidade Federal do Minas Gerais; BrasilFil: Azevedo, Vasco. Universidade Federal do Minas Gerais; BrasilFil: Langella, Philippe. Institut National de la Recherche Agronomique; FranciaFil: Bermudez Humaran, Luis G.. Institut National de la Recherche Agronomique; FranciaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentin

Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

BACKGROUND: Lactic acid bacteria (LAB) are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins. However, compared to recombinant lactococci, lactobacilli offer some advantages such as better survival in the digestive tract and immunomodulatory properties. Here, we compared different strategies to optimize the production of bovine β-lactoglobulin (BLG), a major cow's milk allergen, in the probiotic strain Lactobacillus casei BL23. RESULTS: Using a nisin-inducible plasmid system, we first showed that L. casei BL23 strain could efficiently secrete a reporter protein, the staphylococcal nuclease (Nuc), with the lactococcal signal peptide SP(Usp45 )fused to its N-terminus. The fusion of SP(Usp45 )failed to drive BLG secretion but led to a 10-fold increase of intracellular BLG production. Secretion was significantly improved when the synthetic propeptide LEISSTCDA (hereafter called LEISS) was added to the N-terminus of the mature moiety of BLG. Secretion rate of LEISS-BLG was 6-fold higher than that of BLG alone while intracellular production reached then about 1 mg/L of culture. The highest yield of secretion was obtained by using Nuc as carrier protein. Insertion of Nuc between LEISS and BLG resulted in a 20-fold increase in BLG secretion, up to 27 μg/L of culture. Furthermore, the lactococcal nisRK regulatory genes were integrated into the BL23 chromosome. The nisRK insertion allowed a decrease of BLG synthesis in uninduced cultures while BLG production increased by 50% after nisin induction. Moreover, modification of the induction protocol led to increase the proportion of soluble BLG to around 74% of the total BLG production. CONCLUSION: BLG production and secretion in L. casei were significantly improved by fusions to a propeptide enhancer and a carrier protein. The resulting recombinant strains will be further tested for their ability to modulate the immune response against BLG via mucosal delivery in a cow's milk allergy model in mice